SKU: AK-AB-001

Format: Up to 320 Tests (5 Sensor Strips included)
Assay Format: Inverse occupancy using Protein A capture

Built for consistent, quantitative analysis of human IgG, this kit uses Protein A affinity capture and an inverse occupancy signal format. The design supports accurate performance across a broad dynamic range, even in crude cell culture supernatants.

With strong reproducibility and reduced setup burden, it’s ideal for antibody screening and early-stage process development.

This kit uses a dip-sensor format with Protein A pre-coating. During incubation, antibodies bind via Fc interaction, followed by detection of unoccupied binding sites to generate an inverse signal.

Assay Workflow Schematic. (1) Sensor surface coated with Protein A or Protein G. (2) Antibody (analyte) binds via Fc interaction. (3) Detection molecule binds remaining unoccupied Fc-binding sites, generating an inverse signal.

This assay supports reproducible mAb quantification across high, mid, and low concentration ranges. Performance is maintained in both clarified and crude matrices, ideal for decision-making in clone selection, screening, or upstream development.

Representative standard curves across high, mid, and low ranges (human IgG1).

This kit includes all required reagents and materials for up to 320 antibody quantification tests.

Safety Data Sheets (SDS) are available for each component.

• Regeneration Buffer | Antibody Quantification (A1-8022)
• Wash Buffer | Antibody Quantification (A1-8023)
• Sample Dilution Buffer | Antibody Quantification (A1-8024)
• Detection Reagent | Antibody Quantification (A1-8025)
• Substrate (A1-8026)