His-tagged proteins are widely used in expression and purification workflows, but quantifying them across different sample types and buffers can be challenging.
This application note presents a premix-format assay run on the Amperia™ platform, enabling direct measurement of His-tagged proteins from crude lysates without the need for purification or specialised equipment.
Highlights
- Works with imidazole-containing buffers
- Compatible with crude lysates, supernatants, and in-process samples
- Ideal for construct screening, expression monitoring, and purification steps
- Powered by Amperia’s premix detection workflow and intuitive interface
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Tags: His-tag, Expression Systems, Protein Purification