Reliable concentration measurement underpins confident development decisions.
Monoclonal antibodies (mAbs) play a central role in biologics development — from early discovery to clinical manufacturing. Whether you’re screening clones, monitoring upstream expression, or evaluating purification yield, accurate quantification is essential for making informed decisions.
Why accurate mAb quantification matters
Consistent measurement of antibody concentration enables:
- Monitoring productivity across cell lines, constructs, or timepoints
- Assessing purification performance and recovery
- Normalising input for binding, activity, or stability assays
- Ensuring comparability across batches or process conditions
Poor quantification early in development can lead to misleading results, while inconsistency in later stages can affect formulation, dosing, or quality control studies.
Challenges with conventional methods
Standard colorimetric assays (e.g. BCA, Bradford) measure total protein but lack specificity. They cannot distinguish antibodies from host cell proteins or media components — a significant limitation in crude samples or early-stage processes.
ELISAs offer higher specificity but introduce other challenges:
- Require validated capture and detection antibodies
- Often involve lengthy, multi-step protocols
- Prone to variability across plates, operators, or labs
Where Amperia™ fits
The Amperia™ Antibody Quantification Kit uses Redox Electrochemical Detection (RED) to directly measure antibody concentration based on binding to a functionalised sensor surface.
Once bound, the antibody participates in a surface enzymatic reaction that generates an electrochemical signal. This signal is compared against a standard curve to determine protein concentration, with response characteristics defined by the assay format.
Key benefits:
✔ Compatible with a wide range of sample matrices, including culture supernatants
✔ Suitable for clone screening, upstream monitoring, and purification tracking
✔ Flexible throughput and software-guided setup for consistent results
Amperia™ helps eliminate bottlenecks in quantification, enabling faster, more confident decision-making across your mAb workflows.